Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides.

Glucose 6-phosphate dehydrogenase from Zeuconostoc mesenteroides, previously isolated in crystalline form (Olive, C., and Levy, H. R., Biochemistry, 6, 730 (1967)), is shown to be essentially homogeneous by disc gel electrophoresis and sedimentation velocity analysis. The weight average molecular weight is 103,700, determined by both high speed and low speed sedimentation equilibrium techniques at pH 4.6, which is the approximate isoelectric point. The partial specific volume was determined to be 0.717 at this pH. The enzyme possesses an absorption spectrum showing a maximum at 280 5 run with E&iTb = 1.15 and considerable fine . structure in the region of 250 to 270 nm.